胰岛素样生长因子-1受体在恶性黑色素瘤中的表达及临床意义

Expression and Clinical Significance of Insulin-like Growth Factor 1 Receptor in Malignant Melanoma

目的探讨恶性黑色素瘤组织中胰岛素样生长因子-1受体(insulin-like growth factor-1receptor,IGF-1R)的表达及其临床意义。方法选取经病理检查明确诊断的恶性黑色素瘤石蜡切片26例(恶性黑色素瘤组)及正常色素痣石蜡切片20例(正常色素痣组)。采用EnVision两步法,以兔抗人IGF-1R多克隆抗体检测上述两组IGF-1R的表达情况,并分析恶性黑色素瘤组织中IGF-1R的表达与年龄、性别及有无远处转移的关系。结果恶性黑色素瘤组IGF-1R的阳性表达率为57.7%(15/26),正常色素痣组为25.0%(5/20),两组比较差异有统计学意义(χ2=4.912,P=0.023);恶性黑色素瘤组IGF-1R的高表达与有无远处转移相关(P=0.038),而与性别、年龄无关(P=0.453,P=0.701)。结论 IGF-1R的表达量可以预测原发性恶性黑色素瘤的转移及预后,对恶性黑色素瘤的治疗可能具有一定的指导意义。

Objective To investigate the expression and clinical significance of the insulin-like growth factor-1 receptor（IGF-1R）in malignant melanoma.Methods 26 cases of paraffin sections diagnosed as malignant melanoma by pathological examination（malignant melanoma group）and 20 paraffin sections of normal pigmented nevus（normal pigmented nevus group）were selected.With En Vision two-step method,the expression of IGF-1R in the two groups was detected by rabbit polyclonal anti-human IGF-1R antibody,and relationship of IGF-1R expression with age,sex and distant metastasis was analyzed.Results The positive expression rate of IGF-1R in malignant melanoma group was 57.7%（15/26）,significantly higher than that in normal pigmented nevus group（25.0%,5/20）,the statistical analysis showed significant difference（χ2=4.912,P=0.023）;the high IGF-1R expression in malignant melanoma group was related with distant metastasis（P=0.038）,but not with sex and age（P=0.453,P=0.701）.Conclusion IGF-1R expression level can predict the metastasis and prognosis of primary malignant melanoma,and may have some significance for its treatment.