杀虫基因高效植物表达载体的构建

Construction of insect-resistant gene plant expression vector

以质粒pCAMBIA1200、pBI121、pMMAR和pC1300MAR为基础,通过酶切连接,构建植物中间载体pC2MARHyg;再利用pAVAc质粒、载体pGA1611和中间载体pC2MARHyg,构建含有Ubi-1启动子、顺向重复MAR和杀虫基因AVAc的高效植物表达载体pC2MAR-AVAc,然后转化农杆菌EHA105,经筛选鉴定获得阳性克隆,为进一步开展植物抗虫基因工程研究奠定基础。

Based on plasmids of pCAMBIA1200,pBI121,pMMAR and pC1300MAR,plant intermediate vector pC2MARHyg was constructed by digestion and ligation.Plant expression vector pC2MAR-AVAc containing Ubi-1 promoter,direct duplication MAR sequence and insect-resistant gene（AVAc）,were constructed using pAVAc plasmid,pC2MARHyg vector and pGA1611 vector.The constructed vector was transformed to Agrobacterium EHA105.The newly constructed plant expression vector can be used in molecular breeding programs tailored for developing insect-resistance crop plants.