摘要
目的研究原发性肝细胞癌(HCC)中CyclinD1基因的表达及其与p53基因、肝癌细胞增殖活性的关系,探讨其在HCC的发生、发展中可能的作用。方法应用免疫组织化学及定量DNA图像分析的方法,检测CyclinD1、P53基因在32例HCC和6例正常肝组织中的表达,并分析cyclinD1蛋白的表达与肝癌细胞增殖活性的关系。结果肝癌组织中cyclinD1蛋白的过表达率明显高于癌旁肝组织(71.8%,23/32对0%,0/32;P<0.01)。cyclinD1蛋白的表达与HCC的P53基因表型状况及其它临床病理学指标无关。HCC中S期细胞比例在不同cyclinD1蛋白水平的HCC中未见显著性差异(P>0.05)。结论CyclinD1基因的过度表达可能参与HCC的发生、发展。cyclinD1蛋白的免疫组化染色可作为HCC的辅助病理学诊断指标之一。
Objective To study the CyclinD1 gene expression in human hepatocellular carcinoma (HCC) and its clinical pathological significance. Methods By sing immunogistochemistry,the expression of cyclinD1 and p53 in 32 HCC samples and 6 normal liver tissues was detected respectively.With quantitative DNA examination,it was also analyzed the relationship between the aberrant expression of cyclinD1 and the proliferation of HCC. Results The expression of cyclinD1 in HCC was much higher than that in paracercinoma liver tissues (71.8% 23/32 vs.0% 0/32, P <0.01).However,no significant difference in the expression of cyclinD1 in HCC samples was found in different p53 status and other clinical pathological parameters. Conclusion The over expression of cyclinD1 may participate in the carcinogenesis and progression of HCC.The cyclinD1 may be a tumor marker of HCC.
出处
《肝胆外科杂志》
1999年第3期214-216,共3页
Journal of Hepatobiliary Surgery