浓缩DNA法结合miniSTR分型技术检验微量DNA

Forensic Analysis of LCN DNA Using Sample Concentration Methods Followed by miniSTR Genotyping

目的优化低拷贝数DNA STR分型方法。方法对采用磁珠法或Chelex-100法提取DNA,Identi-filer试剂盒扩增,未获得分型结果的日常检案检材,采用物理浓缩法或过柱浓缩法浓缩DNA,采用miniFilerTM试剂盒再次扩增分型。结果 127例检材中,47例磁珠法提取DNA未获得分型的样品,分型成功率为36%;80例Chelex-100法提取DNA未获分型的样品,分型成功率为30%。结论采用浓缩法和miniFilerTM试剂盒,可以提高日常检案中低拷贝数检材的STR检验分型成功率。

Objective To optimize low copy number（LCN） DNA analysis methods for forensic STR genotyping.Methods Two groups of DNA sample,extracted using either Magnetic bead method or Chelex-100 methods,were previously amplified with a Identifiler PCR Amplification kit,but no genotype was detected.The DNA samples were concentrated using either a drying method or the Microcon-100 method,then am-plified using an miniFilerTM PCR Amplification kit and genotyped.Results Among the 127 DNA samples,47 samples,previously extracted using the Magnetic bead method,were genotyped with 36% success rate.Eighty samples,previously extracted using the Chelex-100 method,were genotyped with 30% success rate.Conclusion The application of sample concentration methods and miniFilerTM kit can improve the success rate of LCN STR analysis.