三种方法测定肌钙蛋白Ⅰ分析性能的比较与评价

Performances of Three Methods for the Detection of Troponin-Ⅰ

用化学发光法、胶体金法及胶乳增强免疫比浊法测定60例肌钙蛋白I(cTnI),评价三种方法的分析性能及测定结果的差异及相关性,以便临床根据需要选择合适的方法测定cTnI。通过本次评价发现,胶体金法及化学发光法与临床诊断有很好的总符合率,且灵敏度、特异性较高,误诊率和漏诊率低。三种检测方法测定结果之间有显著性差异,P<0.001,两两比较,胶乳法与胶体金法、化学发光法之间有显著性差异,P值均<0.001;胶体金和化学发光法之间没有显著性差异,P=0.986。胶体金法(Y1)与化学发光法(X)相关性好,Y1=1.20X-0.11,R2=0.933;相关性优于胶乳增强免疫比浊法(Y2),Y2=3.65X+0.49,R2=0.892。在分析性能上胶体金法测定cTnI优于胶乳增强免疫比浊法,且与化学发光法有较好的相关性,适用于急诊及床旁检测。

To evaluate the performance of three different methods on the detection of Troponin-Ⅰ,the serum Troponin-Ⅰ levels in 60 samples were detected by chemiluminescence immunoassay（CLIA）,Colloidal gold colloid immunochromatography assay（CGCIA） and latex enhanced immunoturbidimetric assay（LEITD） respectively.The results showed that the results measured by CLIA and CGCIA matched with clinical diagnosis verification very well,with high coincidence,sensitivity,and specificity.The results measured by three methods had significant difference（P0.001）.When compared with each other,the results between CLIA and CGCIA have no differences,while the results of CLIA and CGCIA between LEITD were different（P0.001）.The results measured by CGCIA were better correlated with CLIA.The performance of CGCIA is better than LEITD,especially in the emergency clinical laboratory and Point of Care Testing.