壳聚糖—硅胶复合载体固定化乙醇脱氢酶技术研究

Research on immobilized of alcohol dehydrogenase with carrier of compound silica

为提高乙醇脱氢酶使用率,以涂敷壳聚糖的硅胶为载体、戊二醛作为交联剂,采用吸附法与交联法相结合方法对乙醇脱氢酶进行固定化,并以酶活力、相对酶活、活力回收率为指标;结果表明:以制备复合硅胶为载体,选择1%戊二醛为交联剂,载体与酶以1∶1(w/v)比例振荡交联2 h,酶活力回收率可达75%,重复使用10次后,酶活力仍可保留28%以上。

To improve the utilization of alcohol dehydrogenase , the silica coated with chitosan is used as compound carrier and glutaraldehyde as crosslinker, by combination of adsorption and cross-linking method to immobilize alcohol dehydrogenase. To enzyme activity, relative activity, activity recovery as an index, the result showed that pectinase activity could retain up to 75% when prepared with compound silica , 1% glutaraldehyde and cross-linking 2h at 1 ： 1 （ w/v ）ratio .The recovery activity of immobilized pectinase was more than 28% after repeated 10 times.