絮凝性酵母IFFI 1792在酒精连续发酵中的应用

Application of Flocculating Yeast IFFI 1792 In Continuous Ethanol Fermentation

絮凝性酵母菌株IFFI 1792被用于酒精连续发酵的研究。连续发酵试验在单级和串联的气升式内循环生物反应器中进行,实现了高细胞浓度高强度酒精连续发酵。试验研究了各种因素对酒精连续发酵的影响,发现溶解氧浓度在一定范围内对高效发酵有利。在单级气升式生物反应器中,生产强度可达30乙醇/L·h;临界生产强度在菌体浓度为80g/L时达到24.5g乙醇/L. h。在两级串联反应器中,生产强度在达到23.5g乙醇/L·h,对应于最终酒糟浓度7.22％(V/V)。连续发酵试验进行到40天,未见发酵活力下降。由试验结果归纳建立了一个非结构的无生长因素的发酵动力学模型,用于连续酒精发酵过程的理论分析。

Flocculating yeast strain IFFI 1792 was used in the studies on continuous ethanol fermentation. Air lift reactors (ALR) were used both in single and in double in series forms. High cell density and productivity were achieved in both cases. In single ALR, the highest productivity reached to 30g ethanol/L/h. In double ALRs,23.5g ethanol/L/h was able to be reached with ethanol concentration of 7.2% (v/v)and 90% ethanol yield.An unstructured dynamics model without growth factors was derived for the process analyses.