铁皮石斛的快速繁殖

Micro-propagation of Dendrobium catenatum Lindley

优化了铁皮石斛组培苗快繁生产过程中的各阶段配方,获得了优质种苗。各阶段最佳培养基组成如下:(1)原球茎增殖:1/2MS+6-BA2mg/L+NAA0.2mg/L+CM100mg/L+糖25g/L+卡拉胶8.6g/L,增殖率达5倍;(2)原球茎分化:MS+BA1mg/L+NAA0.1mg/L+土豆20g/L+糖30g/L+卡拉胶8.6g/L,分化率达85%以上;(3)壮苗、生根:B5+IBA2mg/L+香蕉30g/L+糖30g/L+卡拉胶8.6g/L,生根率达100%。

Media for micro-propagation of Dendrobium catenatum in different stages are optimized. High-quality plantlets and seedlings are produced with the following media： for PLBS proliferation （1/2MS＋6-BA 2 mg/L＋NAA 0.2 mg/L＋CM 100 mg./L＋S 25 g/L＋carrageenan8.6 g/L）, with a proliferation rate of 5 times; for differentiatio （MS＋BA 1 mg/L＋/NAA 0.1 mg/L＋potatoes puree 20 g/L＋S 30 g/L＋ carrageenan 8.6 g/L）, with a differentiation rate of 85%; for both seedling and rooting（Bs＋IBA 2mg/.L＋ banana puree 30 g/L＋ S 30 g/L ＋ carrageenan 8.6 g/L） , with a rooting rate of 100%.