PPARγ激动剂罗格列酮钠对2型糖尿病大鼠肾保护作用的实验研究

Renal protective effects of PPARγ agonist in type 2 diabetic rats

目的探讨过氧化物酶体增殖激活受体γ(PPARγ)激动剂罗格列酮钠(RSG)对2型糖尿病大鼠是否具有肾脏保护作用。方法 30只SD大鼠,随机分成正常对照组(n=10)、糖尿病模型组(n=10)和RSG治疗组(n=10)。糖尿病模型组和RSG治疗组予高糖高脂饮食,并结合小剂量链脲佐菌素(STZ),成功诱导2型糖尿病大鼠模型。造模成功后,RSG治疗组以RSG无菌水混悬液灌胃。4周后观察各组大鼠血清胱抑素C(CystatinC)、尿素氮(BUN)、血肌酐(Cr)、24h蛋白尿定量、血糖等生化指标并宰杀测量肾重/体质量,并加以比较。结果与糖尿病模型组相比,RSG治疗组大鼠血糖、血清胱抑素C及24h尿蛋白定量均明显降低(P均<0.05),但仍高于正常对照组(P均<0.05)。RSG治疗组肾重/体质量比值、TC及TG水平均较糖尿病模型组低(P均<0.05),与对照组比较无明显差异(P均>0.05)。3组BUN、Cr无明显变化(P>0.05)。结论 PPARγ激动剂(RSG)能明显减少2型糖尿病大鼠血糖、尿蛋白及血清胱抑素C水平,对2型糖尿病大鼠肾脏有保护作用。

Objective To explore the protective effects of peroxisome proliferator-activated receptor gamma （PPARγ） agonist （rosiglitazone sodium, RSG） on kidney of rats with type 2 diabetes. Methods Thirty SD rats were randomly divided into three group：control group （n = 10） ,diabetes model group （n = 10） and RSG group （n = 10）. The rats in the diabetes model group and RSG group were administered with high sugar and high fat diet and were given low dose streptozocin （STZ）. After the models of diabetes were successfully made, the rats in RSG group were administered sterilized suspension containing RSG by gastric perfusion for 4 weeks. The serum Cystatin C ,blood urea nitrogen （BUN） ,serum creatinine （Cr） ,24 h uric albumen and blood glucose were detected after four weeks, and the weight of kidney/body mass were measured after killing rats subsequently. Results Compared with diabetes model group, the levels of blood glucose, Cystatin C and 24 h uric albumen were obviously lower （ all P 〈 0.05 ） in RSG group, while were still higher than those in control group （ all P 〈 0. 05 ）. The weight of kidney/body mass ,the levels of total cholesterol and triglyceride in RSG group were lower than those in diabetes mod- el group （ all P 〈 O. 05 ）, but there was no significant difference between RSG group and control group （ all P 〉 0. 05 ）. The levels of BUN and Cr had no statistical difference among three group （ all P 〉 0. 05 ）. Conclusions RSG could decrease the levels of blood glucose, Cystatin C and 24 h uric albumen, and thereby has protective effects on the kidney of rats with type 2 diabetes.