胃癌、消化性溃疡患者幽门螺杆菌和iceA基因的研究

Investigation of cagA, iceA gene in Helicobacter pylori strains in thepatients with gastric carcinoma and peptic ulcer

目的研究幽门螺杆菌（Ｈｐ）的ｉｃｅＡ基因与胃癌、消化性溃疡病的关系。从１２１例患不同胃十二指肠疾病的患者胃活检组织中分离培养Ｈｐ，ＰＣＲ扩增检测Ｈｐ的ｃａｇＡ和ｉｃｅＡ基因。结果胃癌、消化性溃疡及功能性消化不良患者Ｈｐ的ｃａｇＡ基因的阳性率分别为：９１２％、８７１％和８９７％（Ｐ＞０．０５）；而ｉｃｅＡ１基因的阳性率分别为：６８０％、７１０％和６９２％（Ｐ＞０．０５）。结论我们的研究表明Ｈｐ的ｃａｇＡ和ｉｃｅＡ１基因与胃癌及消化性溃疡的发生不相关。

Aim To investigate the possible role of cagA,iceA gene in Helicobacter pylori in the pathogenesis of gastric carcinoma and peptic ulcer. Methods:XH.pylori strains were isolated from 121 patients with gastric carcinoma(20),peptic ulcer(62)and nondyspepsia(39).cagAand iceA genes status were measured using polymerase chain reaction. Results: The prevalence of cagA gene in H.pylori strains from gastric carcinoma,peptic ulcer and nondyspepsia was 91.2%,97.1% and 89.7%(P>0.05),respectively,while the frequency of iceA1 gene was 68.0%,71.0% and 69.2%(P>0. 05),respectively. Conclusion: There was no association betweencagA and iceA1 genes in H.pylori strains and the pathogenesis of peptic ulcer or gastric carcinoma in our population.