摘要
目的：探讨肝门部胆管癌组织中血管内皮细胞生长因子（ＶＥＧＦ）和血管生成的相关性。方法：应用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）和免疫组化技术对２６例肝门部胆管癌、癌周组织及１２例正常组织中ＶＥＧＦｍＲＮＡ和蛋白及微血管密度（ＭＶＤ）进行了检测。结果：２６例肝门部胆管癌组织中ＶＥＧＦｍＲＮＡ阳性表达率为７６．９％（２０／２６）；癌周组织阳性表达率为２８．９％（７／２６）；正常组织表达率为８．３％（１／１２），三者差异有显著性（Ｐ＜０．０１）。ＶＥＧＦｍＲＮＡ阳性表达与ＶＥＧＦ蛋白表达具有一致性；ＶＥＧＦｍＲＮＡ阳性者ＭＶＤ值显著高于阴性者（Ｐ＜０．０１）；ＶＥＧＦｍＲＮＡ表达和ＭＶＤ与肝门部胆管癌的分化程度、浸润转移密切相关（Ｐ＜０．０５）；而与发生部位、病理类型、肿瘤大小、临床分型无关（Ｐ＞０．０５）。结论：ＶＥＧＦ在肝门部胆管癌发生和浸润转移过程中发挥重要作用，肿瘤血管生成与肝门部胆管癌浸润转移密切相关。
Objective :To
elucidate the relationship of vascular endothelial growth factor and angiogenesis in the
progression of hilar cholangiocarcinoma. Methods: VEGF mRNA and microvessel
density(MVD)in 26 cases of hilar cholangicarcinoma and 12 cases normal bile duct tissue were
examined with reverse transcriptasepolymerase chain reaction(RTPCR)and
immunohistochemical stain. Results: VEGF mRNA Expression in tumor tissue was found in
76.9%(20/26)of patients,whereas,it was seen in 26.9%(7/26)of nontumor bile duct and
8.3%(1/12)normal bile duct ( P <0.01),MVD was significanty higher in VEGF mRNA positive hilar
cholangiocarcinoma than that in VEGF mRNA negative.VEGF mRNA expression and MVD
correlated with differentiation,invasion metastasis ( P <0.05),but didnt relate with clinical
type,pathological type,size and site( P >0.05). Conclusions: VEGF mRNA is highly related to
angiogenesis of hilar cholangiocarcinoma and promotes its growth,invasion and
metastasis;angiogenesis may play an important role in the pathogenesis and progression in
hilar cholangiocarcinoma.
