摘要
目的：研究全身热疗对机体的损伤、与热剂量的关系及全身热疗热耐受现象的量化。方法：分别以４０５℃、４１０℃、４１５℃、４２０℃、４２５℃、４３０℃，不同时间对ＮＩＨ小鼠进行加温治疗，观察其死亡情况。热耐受实验以４１５℃２５分钟作为引导加温，然后每隔２４小时按不同温度－时间进行治疗，记录其死亡率。结果：４１５℃、４２℃、４２５℃和４３℃不同加温时间的半数致死时间分别为４０８１ｍｉｎ、２２５２ｍｉｎ、１８２３ｍｉｎ及１３７８ｍｉｎ。热耐受研究证实热疗后２４小时最强，之后每２４小时消减近５０％，但４１５℃２４小时热耐受接近无穷大。４０５℃加热至３小时未见有死亡，４１℃产生最大死亡率为４０％。结论：小鼠全身热疗４１５℃以下约２小时后产生热耐受；２４小时产生最大热耐受，每２４小时约递减５０％。
Objective: To study the correlation of thermodose and death rate of
mice and quantitative analysis of thermotolerance in whole body hyperthermia (WBH).
Methods: NIH mice were used in our experiments. Death was the end point of the observation.
Treatment temperature ranged from 40.5 to 43. Thermotolerance was induced with a prior-shock
of 25 min at 41.5.Thermotolerance at 41.5 and 42 was measured at 24 hr.,72 hr. and 96 hr. after
the challenge dose. Results: Time required to kill 50% mice were 40.81 min. at 41.5,22.52 min.
at 42, 18.23 min. at 42.5and 13.78 at 43.Thermotolerance reached maximum at 24 hr. and
decreased by 50% every 24 hrs, Maximal death rate was 40% at 41. Conclusions
:Thermotolerance is induced after 2 hrs. at temperature<41.5 during treatment. Thermotolerance
is maximal at 24 hr. and decreased by about 50% every 24 hrs.
基金
国家人事部博士后研究项目
关键词
全身热疗
热耐受
热疗法
肿瘤
whole body
hyperthermia
NIH mice
thermotolerance
