摘要
目的探讨细菌鞭毛银染法中的细菌最佳培养条件。方法枯草杆菌在30℃和35℃的营养琼脂、血液琼脂及肉汤培养基中培养6~12h,分别在6、8、10、12h取细菌涂片,并使用银染法染色,显微镜下观察鞭毛。结果在30℃的营养琼脂斜面中培养枯草杆菌8~10h后,采用银染法进行鞭毛染色,在显微镜下观察到视野背景干净,鞭毛数量较多且形态完整,染色清晰。结论银染法进行鞭毛染色时,宜在30℃营养琼脂斜面中培养枯草杆菌8~10h后,染色效果最好。
Objective To investigate the best cultivated condition of bacillus subtilis in silver-staining. Methods Bacillus subtilis was cultivated with 30 ℃,35 ℃ nutrient agar,blood agar and bouillon for 6 ~ 12 h,and taken to smear at 6,8,10,12 h each. Bacterial flagella was observed with micro after argentation. Results Many integrity flagella and clean vision were observed after raising bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and staining. Conclusion To cultivate the bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and stain have the best result.
出处
《临床医学工程》
2010年第10期40-41,共2页
Clinical Medicine & Engineering
关键词
鞭毛
银染法
培养
Flagellum
Silver-staining
Cultivation