小肠结肠炎耶尔森菌菌种保存22年的跟踪调查

Investigation of the long-tearm preservation method of Yersinia enterocolitica

目的为了探索一种简便、实用并能长期保存小肠结肠炎耶尔森菌菌种的方法。方法 1988年将小肠结肠炎耶尔森菌菌种(血清O:3型和血清O:9型)接种于鸡蛋斜面培养基及半固体培养基复苏后加灭菌液体石蜡,置4℃普通冰箱保存,且每年进行复苏鉴定。结果 O:3型菌种可保存21年,O:9型可保存22年以上,O:9型到发稿为止,依然存活。结论鸡蛋斜面保存法和半固体穿刺法-4℃保存小肠结肠炎耶尔森菌菌种效果良好,可长时间保存,且不需要反复传代。

Objective To explore a simple,practical and long-term preservation method of Yersinia enterocolitica.Methods Yersinia enterocolitica strains（Serotype O：3 and Serotype O：9）were inoculated into egg slant medium and semi-solid medium for resuscitation,then the strains were preserved by sterile liquid paraffin in the refrigerator at 4 ℃ in 1988 and the strains were selected and tested every year.Results The Yersinia enterocolitica Serotype of O：3 can be stored for 21 years.Serotype of O：9 can be stored more than 22 years,and it still survives at the time when the article is published.Conclusion The egg slant medium preservation and semi-solid liquid paraffin in the referigerator at-4 ℃ are attended with good results for Yersinia enterocolitica.The strains of Yersinia enterocolitica can be preserved by these two methods for a long time,and need＇t repeated passage.