兔角膜碱烧伤后角膜组织中NO、MDA含量及SOD活性的变化

The Changes of Nitric Oxide and MDA Levels and SOD Activityin Corneal Tissue After Corneal Burn by Alkali in Rabbits

目的：探讨一氧化氮与脂质过氧化反应在角膜碱烧伤中的作用。方法：在兔右眼角膜用２ｍｏｌ／ＬＮａＯＨ溶液烧伤６０秒钟后，分别于第１、４、８、１６、３２及６４天测量其ＮＯ和丙二醛（ＭＤＡ）的含量及ＳＯＤ活性，左眼为正常对照。结果：烧伤后的第１、４、８、１６及３２天，其ＮＯ和ＭＤＡ含量显著高于对照组，至第６４天接近对照组水平。烧伤后第１～１６天，ＳＯＤ活性较对照组显著降低，第３２天已基本恢复至对照组水平。结论：角膜碱烧伤后角膜组织中ＮＯ水平升高和脂质过氧化反应增强可能是其损伤机制之一。

Objective To explore the effects of nitric oxide and lipid peroxidation in the cases of corneal alkali burn. Method Burn the right eye cornea of rabbits with 2 mol/L NaOH solution for 60 seconds while the left eyes as control group,and then measure the NO level,MDA level and SOD activity in cornea tissue. Results On the lst,4th,8th,16th and 32 nd days after the burning,the NO and MDA level of experimental group were markedly higher than those of control group.After 64 days,these two indexes of experimental group came near those of the control.From the lst to 16th days,SOD activity of experimental group was remarkably decreased;on the 32nd day,no noticeable difference was found in both groups and on the 64th day,it reverted to the control level. Couclusion After corneal alkali burn,it was possibly one of the machanism of injury that NO level increases and lipid peroxidation strengthens in damaged corneal tissue.