黏蛋白1在肝内胆管结石合并肝内胆管癌的表达及意义

Expression and significance of MUC1 in hepatolithiasis associated with intrahepatic cholangiocarcinoma

目的探讨肝内胆管结石合并肝内胆管癌组织黏蛋白1(MUC1)的表达及其临床意义。方法选择1989年8月至2009年6月手术切除肝组织石蜡标本90例,肝内胆管结石合并肝内胆管癌33例为实验组,肝内胆管结石32例为对照组,肝脏良性肿瘤瘤旁1～2cm正常肝内胆管组织25例为空白组。各取组织切片进行MUC1免疫组化染色,分析其表达差异及意义。结果 MUC1在实验组、对照组及空白组的阳性率分别为54.5%(18/33)、28.1%(9/32)和0(0/25),3组间比较,P<0.01;MUC1阳性表达在病理组织学分级、淋巴结转移的差异均有统计学意义(P分别<0.05,<0.01);肿瘤患者MUC1阳性表达者的累积生存率比阴性表达者低(P<0.01)。结论 MUC1在肝内胆管结石发展为胆管癌过程中可能起重要作用,MUC1高表达与肝内胆管结石合并肝内胆管癌的恶性程度以及预后有关。

Objective To investigate the role of MUC1 in hepatolithiasis associated with intrahepatic cholangiocarcinoma.Methods Ninety cases biopsies of hepatectomies performed from August 1989 to June 2009 in the second affiliated hospital of Sun Yat-sen university were stained immunohistochemically for MUC1.Among them 33 cases with hepatolithiasis associated with intraheptic cholangiocarcinoma were experimental group, 32 cases with hepatolithiasis were control group and 25 cases of normal intrahepatic bile ducts were taken 1-2 cm distal to benign hepatic neoplasm as blank group.The immunohistochemical staining of MUC1 were performed by tissues slice.Results MUC1 positive rate were 54.5%（18/33）, 28.1%（9/32） and 0 （0/25） in experimental group,control group and blank group respectively, P〈0.01.The positive rates were significantly different in experimental group among histopathologic classification （P〈0.05） and whether with lymph node metastasis or not （P〈0.01）.The accumulated survival rate was lower in MUC1 expressed tumor patients than that in MUC1 non-expressed tumor patients, P〈0.01.Conclusions MUC1 probably plays an important role during intrahepatic cholangiocarcinogenesis.High level expression of MUC1 is related with malignancy of intrahepatic cholangiocarcinoma and with worse prognosis.