摘要
目的探讨重症急性胰腺炎(SAP)时胰腺组织一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)表达的变化。方法 36只健康雄性Sprague-Dawley大鼠随机分为2组。SAP组(n=18)以逆行胰胆管内加压注射5%牛磺胆酸钠的方法建立SAP模型;对照组(n=18)以同法注射等剂量生理盐水。造模后6、12、24 h每组随机处死大鼠6只,检测血淀粉酶、胰腺组织病理组织学改变、胰腺组织NO水平及iNOS活性以及腺腺组织iNOS mRNA表达的变化。结果与对照组相比,SAP组在6 h时间点上,胰腺组织中NO水平、iNOS活性明显升高(P<0.01),免疫组化和RT-PCR结果也显示iNOS蛋白及iNOS mRNA表达增高(P<0.01);随时间延长,在12 h和24 h各指标到达高峰,24 h仍维持在较高水平(P<0.01)。结论在SAP时,随着时间的延长,iNOS表达增高,从而产生高浓度的NO,加重胰腺组织的损伤。
Objective To investigate the changes in the formation of nitric oxide（NO） and the expression of inducible nitric oxide synthase（iNOS） in pancreatic tissue in severe acute pancreatitis（SAP） in rats.Methods Thirty-six healthy male Sprague-Dawley（SD） rats were randomly allocated into sham-operated group（control group） and SAP group（n=18 each）.The model of SAP was established by injection of 5% sodium taurocholate into the common choledocho-pancreatic duct in SD rats.The same dosage of normal saline was given to the control group in the same way.The rats were randomly sacrificed at 6 h,12 h and 24 h,respectively（n=6 each）,and their blood samples were collected for analyses.The variation of serum amylase levels and the pathohistological changes of the pancreas were detected by colorimetric method.The expression of iNOS proteins and iNOS mRNA in the pancreas were determined by immunohistochemistry and reverse transcriptase polymerase chain reaction method（RT-PCR）,respectively.Results The NO level and the activity of total iNOS in the pancreas at 6 h significantly increased in the SAP group as compared to the control group（P0.01）.Immunohistochemistry and RT-PCR also revealed an increase in the expression of pancreatic iNOS protein and mRNA in the pancreas（P0.01）.Each parameter increased with time and reached the peak at 12 h and still retained a high level at 24 h（P0.01）.Conclusion In SAP,the iNOS expression was upregulated with time and thus the high concentration of NO was produced,which aggravated the lesion of pancreatic tissue.
出处
《徐州医学院学报》
CAS
2010年第10期648-651,共4页
Acta Academiae Medicinae Xuzhou
