毒死蜱降解菌枝孢霉菌的紫外诱变和筛选

Breeding and Screening of Chlorpyrifos-Degrading Strain Cladosporium cladosporioides by Ultraviolet Ray Mutation

通过紫外诱变毒死蜱降解菌枝孢霉菌Cladosporium cladosporioidesHu-01,利用高效液相色谱(HPLC)检测,筛选获得12株突变菌株,其中突变株Hu-01-4和Hu-01-6的降解效果明显高于初始菌株Hu-01.突变株Hu-01-4和Hu-01-6经过加药斜面传种10代,Hu-01-6的降解率比初始菌株提高11.74%,并且保持良好的稳定性.突变菌株Hu-01-6在28℃、pH6.5培养条件下的生长量最大,降解率最高,且均明显高于初始菌株Hu-01.

Twelve mutants were obtained from chlorpyrifos-degrading strain Cladosporium cladosporioides Hu-01 by UV-induced screening and their degradation capacities were determined by high performance liquid chromatography（HPLC）.The degradation abilities of mutant strain Hu-01-4 and Hu-01-6 were higher than the original strain Hu-01.Furthermore,the mutant strain Hu-01-6 kept a good stability of degradation rate after 10 serial passages on the test-tube medium containing chlorpyrifos,increasing by 11.74% compared with the original strain.Moreover,both the growth and degradation ability of mutant strain Hu-01-6 were higher than original strain Hu-01 under the conditions of 28 ℃and pH 6.5.