摘要
采用色谱柱Kromasil-C18柱（250 mm×4.6 mm,5μm）、流动相为乙腈-水（体积比13∶87）、检测波长为239nm、流速为1.0 mL.min-1、柱温为室温的检测条件建立了复方清开灵注射液中有效成分栀子苷的含量测定方法.结果表明,栀子苷进样量在0.5~25.0μg范围内线性关系良好,相关系数（r）=0.999 9,加样平均回收率为100.3%,相对标准偏差（RSD）为0.73%（n=6）.
This study aimed at establishing a method for content determination of geniposide in compound Qingkailing injection by HPLC.The samples were separated on an Kromasil-C18（250 mm ×4.6 mm,5 μm） column using the mobile phases of acetonitrile-water（φ=13∶87）.Detection was performed under the room temperature,with the detection wavelength set at 239 nm and the flow rate at 1.0 mL/min.The results showed that optimum linear relationships were achieved when measuring Geniposide at 0.5-25.0 μg,with the correlation coefficient at 0.999 9.The average recovery of added samples was 100.3% with a RSD of 0.73%（n=6）.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2010年第4期104-107,共4页
Journal of South China Agricultural University
基金
十一五国家科技支撑计划(2006BAD31B06)
