白介素-10基因启动子区单核苷酸多态性与乙肝病毒感染后的相关性研究

Association analysis of interleukin-10 gene promoter polymorphisms with hepatitis B virus infection consequence

目的:研究白细胞介素-10(IL-10)基因启动子区域-1082、-592位点单核苷酸多态性(SNP)与乙肝病毒感染后临床转归之间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析175例乙型肝炎患者和153例正常对照者IL-10基因启动子区域-592、-1082位点的基因多态性,并结合临床资料进行统计学分析。结果:IL-10-592位点在患者组和正常对照组均发现AA、AC、CC 3种基因型,各基因型在患者组和对照组分布上差异均无显著性意义。而IL-10-1082位点在正常对照组有AA、AG、GG 3种基因型,而患者组只发现AA、AG两种基因型。经统计学分析,在基因型分布上,慢性乙型肝炎组与急性乙型肝炎组、重型肝炎组比较差异均有显著性意义(P<0.001,P<0.05);而与肝硬化组相比差异无显著性意义。另外,肝硬化组与急性乙型肝炎组基因型分布的差异亦有显著性意义(P=0.020)。结论:IL-10-1082A等位基因及AA基因型与乙肝病毒感染及感染后转归相关。

Objective：To investigate the correlation between the single nucleotide polymorphism in the promoter region of interleukin-10（IL-10） gene and the clinical phenotypes of hepatitis B virus（HBV）infection in Chinese population.Methods：The genotypes of-1082 and-592 loci in the promoter region of IL-10 were analysis by polymerase chain-restriction fragment length polymorphism method（PCR-RFLP） in 175 cases of patients with hepatitis B and 153 cases of the control.Results：Genotype AA,AC,CC in loci-592 in the promoter region of IL-10 were found in both patients group and the control group,and the genotypes distribution in two groups had no significant difference（P0.05）.The-1082AA and-1082AG genotype in IL-10 promoter region were only found in the group of patients with hepatitis B,but-1082AA,-1082AG and-1082GG genotypes were all found in the control group.The distribution frequences of-1082A allele and-1082AA genotype in IL-10 promoter region were significantly higher in the group of patients with chronic hepatitis B than the group of patients with acute hepatitis B and the group of patients with sever hepatitis B（P0.001,0.05）.Conclusion：The-1082A allele and-1082AA genotype in IL-10 promoter region may be associated with the clinic transformation after chronic hepatitis B infection.