GSTM1基因多态性与AFB_1-DNA加合物水平的关系

The association between GSTM1 gene polymorphism and AFB1-DNA adducts level

目的探讨解毒酶基因谷胱甘肽S-转移酶M1（Glutathione S-transferase M1,GSTM1）多态性对黄曲霉毒素B1（aflatoxin B1,AFB1）高污染区广西人群AFB1-DNA加合物水平的影响。方法采用竞争性酶联免疫吸附法检测研究对象（966例）AFB1-DNA加合物水平,而GSTM1基因型分析则通过PCR-RFLP来完成。结果研究结果显示GSTM1缺失型（GSTM1-null）与高AFB1-DNA加合物水平相关,其校正风险值（OR）为2.09（95%可信区间,即95%CI值为1.61-2.71）。结论 AFB1在带有易感基因型GSTM1-null的人群更易诱导产生DNA损伤（AFB1-DNA加合物）。

Objective To investigate the effects of Glutathione S-transferase M1（GSTM1） polymorphism on AFB1-DNA adducts among inhabitants highly exposure to aflatoxin B1（AFB1） in Guangxi province. Methods A competitive enzyme-linked immunosorbent assay was used to measure the AFB1-DNA adducts levels in 966 participants, the GSTM1 genotype analysis completed by using PCR-RFLP.Results The data results indicated that GSTM1 deficiency（GSTM1-null） is positively associated with elevated AFB1-DNA adducts（ajusted odds ratio,OR=2.09,95% confidence interval,95%CI=1.61～2.71）.Conclusion AFB1 easily resulted in DNA injuries（AFB1-DNA adducts） among population susceptible to GSTM1-null.