发酵吸附分离耦合生产乳酸链球菌素工艺

Technology of Fermentation Coupled with Adsorption Separation for Nisin Production

为了解除微生物发酵过程中生物高分子乳酸链球菌素的抑制作用,提出发酵吸附分离耦合技术。在研究树脂对乳酸链球菌素吸附性能的基础上,研究了树脂及加入时间对发酵过程中细胞生长和乳酸链球菌素合成的影响。结果表明,D113对细胞生长影响很小,当发酵温度30℃,pH6.25,在100mL发酵液中加入2g树脂,加入时间第5.5h,发酵液中乳酸链球菌素的总效价是5189IU/mL,与不加树脂的对照组相比,发酵吸附分离耦合技术使乳酸链球菌素效价提高了54.9%。

The technology of fermentation coupled with adsorption separation was put forward in order to relieve the inhibition of biomacromolecules nisin in the process of fermentation. The effects of resin and addition time to cell growth and nisin synthesis in the process were studied on the basis of the research on resin＇s adsorption capability to nisin. The results show that D113 has few effects to cell growth. When the suitable technology is fermentation temperature 30 ℃,pH 6.25,resin 2 g to be added to the fermentation broth 100 mL and addition time 5.5 h,the total titer of nisin is 5189 IU/mL. The technology of fermentation coupled with adsorption separation has increased the titer of nisin by 54.9% in contrast with the group without add the resin.