HPLC法测定番荔枝种子中泡番荔枝辛的含量

Determination of Bullatacin in the Seeds of Annona squamosa by HPLC

目的:采用HPLC法测定番荔枝种子中泡番荔枝辛的含量。方法:采用HPLC法测定。色谱柱:HederaODS-3(250 mm×4.6 mm,5μm),流动相为甲醇-水(90∶10),流速为1.0 mL.min-1;检测波长为220 nm,柱温25℃。结果:进样量在2.01～20.1μg范围内时,其质量与色谱峰面积的线性关系良好(r=0.999 8)。泡番荔枝辛平均回收率为99.7%,RSD为2.2%(n=6)。测得2008年和2009年采收的番荔枝种子中泡番荔枝辛的含量分别为1.09%和1.42%。结论:本法简便,灵敏,分离度良好,可用于番荔枝种子中泡番荔枝辛的含量测定。

Objective：To determine the content of bullatacin in the seeds of Annona squamosa by HPLC.Method：The conditions of HPLC method were as follows：Hedera ODS-3（250 mm ×4.6 mm,5 μm）;Mobile phase was composed of methanol-water（90∶10） at the flow rate of 1.0 mL ·min^-1;Detection wavelength was 220 nm;Column temperature was 25 ℃.Results：The calibration curve was linear at the range of 2.01-20.1 μg（r=0.999 8）.The average recovery was 99.7% and the relative standard deviation（RSD） was 2.2%（n=6）.The content of bullatacin in the seeds of Annona squamosa collected in 2008 and 2009 were determined as 1.09 % and 1.42 %, respectively. Conclusion： The method is simple and sensitive, and the resolution is good. It can be used for the content determination of bullatacin in the seeds of Annona squamosa.