Ⅰ型糖尿病大鼠模型制备经验

Experience of Prepation for TypeⅠ Diabetic Rat Model

目的探讨Ⅰ型糖尿病大鼠模型制作方法和注意事项。方法一次性大剂量注射链脲佐菌素(STZ)诱导Ⅰ型糖尿病大鼠模型。结果一般情况观察:大鼠多饮、多食、多尿、消瘦明显。体重:实验组在1、2、4、8、12周和对照组同期组比较有显著差异(P<0.05);实验组内1、2、4、8、12周两两比较有显著差异(P<0.05)。尾静脉血糖:实验组在1、2、4、8、12周和对照组同期组比较有显著差异(P<0.05);实验组诱导前与1、2、4、8、12周比有显著差异(P<0.05)。结论通过腹腔一次大剂量注射STZ(55mg/kg)可以严重损伤胰岛,引起与Ⅰ型糖尿病相似的症状。

Objective To explore the method of establishing typeⅠ diabetic rat model.Methods SD rats were injected intraperitoneally with 55 mg/ kg streptozotocin（STZ） once to induce the typeⅠ diabetes models.Results The rats in experimental groups had typical typeⅠ diabetic symptoms,such as the increase of diet,drinking,urine and the decrease of body weight.Compared with normal groups,the weight of rats was decreased significantly in experimental groups（P〈0.05）.Compared with normal groups,the blood suger of rats in experimental groups was increased significantly（P〈0.05）.There were significant differences between each experimental group（P〈0.05）.Conclusion Rat model with typeⅠ diabetes mellitus could be induced by injecting intraperitoneally high dose STZ into rats.