抗CCP抗体与RF联合检测在RA诊断中的价值

Value of combined d etection of serum anti-cyclic citrullinated peptide antibody and rheumatoid factor in diagnosis of rheumatoid arthritis

目的探讨联合检测抗环瓜氨酸肽(CCP)抗体和类风湿因子(RF)在类风湿关节炎(RA)诊断中的价值。方法抗-ccp采用化学发光(MEIA)法,RF采用散射速率比浊法检测,取70例RA、130例非RA患者血清检测抗-CCP和RF。结果 70例RA患者中抗-CCP阳性54例,RF阳性52例,两项同时阳性64例,同时阴性6例,故抗-CCP、RF及两者联合检测在RA患者中的阳性率分别为77.1%、74.3%和91.4%;130例非RA患者中抗-CCP阳性5例,RF阳性38例,无1例两项同时阳性,故抗-CCP、RF及抗-CCP和RF联合检测在非RA患者中的阳性率分别为3.85%、29.23%和0。经过统计学分析联合检测与单项检测比较,差异有统计学意义(P<0.01),且RA患者抗-CCP与RF阳性率显著高于非RA患者,差异有统计学意义(P<0.01)。结论抗-CCP和RF联合检测可明显提高RA的诊断率。

Aim To evaluate the significance of combined detection of serum anti-cyclic citrullinated peptide antibody （anti-CCP）and rheumatoid factor （RF）in the diagnosis of rheumatoid arthritis （RA）. Methods MEIA and IRN techniques were used to detect serum levels of anti-CCP and RF in 70 patients with RA and 130 patients without RA. Results The positive rates of anti CCP,RF and anti-CCP plus RF in 70 RA patients were 77.1%,74.3%,and 91.4% respectively. While the positive rates of anti CCP,RF and anti-CCP plus RA in patients without RA were 3.85 % ,29. 23% and 0 respectively. Through the analysis of statistics ,the combined examination a s well as the single examination great significance statistically （P〈0. 01）. The positive rate of anti-CCP and RF in RA patient was significantly higher than that of non-RA patient s （P〈0.01）. Conclusion Determination of the combination of serum anti CCP and RF is helpful in the diagnosis of patient s with RA.