补阳还五汤含药血清对缺氧损伤PC12细胞的保护作用

BHD containing serum on PC12 cells hypoxic injury protection

目的:探讨补阳还五汤含药血清对缺氧损伤PC12细胞的保护作用。方法:应用中药复方血清药理学研究方法,制备家兔补阳还五汤含药血清,观察其对缺氧PC12细胞损伤模型细胞形态学、存活力及上清液中PAF含量的影响。结果:PC12细胞缺氧造模后,出现急性损伤,形态变异,补阳还五汤含药血清组表现为自我修复,形态与正常细胞组相似;补阳还五汤含药血清组能提高细胞存活力,降低凋亡率及降低细胞上清PAF含量(P<0.05)。结论:补阳还五汤含药血清对缺氧损伤PC12细胞有明显的保护作用,其机制与降低缺氧损伤细胞PAF含量释放、保护细胞形态和提高存活力有关。

Objective： To investigate the BYHWD containing serum on PC12 cells in hypoxic injury in rats.Methods： The serum pharmacological study traditional Chinese medicine methods,preparation of rabbit BWD containing serum was observed on PC12 cell injury model of hypoxic cell morphology,viability,and PAF content of the supernatant.Results： PC12 cells after the model hypoxia,acute injury,morphological variation,BWD containing serum group showed self-healing,shape and normal cells were similar;BHD containing serum group could increase the cell viability,reduced apoptosis and decreased cell supernatant PAF levels（P0.05）.Conclusion： BWD containing serum on PC12 cells in hypoxic injury has protective effects,the mechanism of cell damage and reduce the oxygen content of the release of PAF,protect and improve the viability of cell morphology on.