D4020大孔树脂提纯发酵液中紫杉醇的研究

The Study of Purification of Taxol from Endophytic Fungi Fermentation Broth by D4020 Macroporous Resin

采用D4020型大孔树脂对红豆杉产紫杉醇内生真菌发酵液的粗提液进行吸附和洗脱试验,同时应用HPLC进行分析检测,确定了最佳工艺条件。结果表明,D4020型吸附树脂对紫杉醇有吸附分离效果。优化工艺条件为:室温下,样品溶于体积分数50%甲醇-水溶液,上柱液体积流量1.5 mL/min,体积分数80%乙醇-水溶液以体积流量0.5 mL/min洗脱,洗脱剂用量为7倍量树脂体积,回收率达90%。

Absorption on D4020 macroporous resin was studied as a method to purify the taxol from endophytic fungi fermentation broth.The optimum conditions for absorption and desorption of taxol was determined and listed as follows： The adsorbed temperature at room temperature,the sample was dissolved in 50 % methanol,and added on a column of the resin at the rate of 1.5 mL/min,then it was washed with 20% ethanol,and was eluted with 7 BV 80% ethanol at 0.5 mL/min speed.Under the optimum conditions,the recovery rate achieved at 90%,so this resin can be used for separation and concentration of taxol.The static absorption dynamic curve and isotherm of taxol on D4020 resin showed that D4020 macroreticular resin was suitable for absorbing and separating taxol.