制作云南乳扇用酸乳清中乳杆菌的多样性分析

The Polymorphism of Lactobacilli from Acid Whey for Dairy Fan in Yunnan Area

针对制作云南乳扇用酸乳清中的乳杆菌的来源及种属,对其多态性进行研究。采用AFLP技术和NTSYS-pc2.1软件对从云南大理白族地区牧民家庭制作乳扇用的的酸乳清中分离出的70株乳杆菌进行多样性分析。结果表明,AFLP扩增出的条带清晰,重复性好,多态性高。聚类分析表明,在0.89的水平上,70株乳杆菌共分为4个大群:高加索乳杆菌群Ⅰ、发酵乳杆菌群Ⅱ,瑞士乳杆菌群Ⅲ和植物乳杆菌群IV。其中,瑞士乳杆菌群Ⅲ中的乳杆菌占供试乳杆菌的65.7%,为优势菌群。进一步对优势菌群聚类,进行种内分型,以0.90为界,群Ⅲ被分为8个基因型。

The target of this study is to investigate the biodiversity of 70 Lactobacilli strains,isolated from the acid whey for dairy fan made by herd man families in Yunnan Dali the Bai nationality Region in China,for this,AFLP technique integrated with NTSYS-pc2.1 software was developed to investigate the polymorphism and types of the strains.The results suggested that the amplified bands of AFLP were clear and stable with high repetition and high polymorphisms.The cluster analysis showed that at level 0.89,70 tested strains were clustered into 4 groups： Lactobacillus fermentum,Lactobacillus helveticus（65.7%） and Lactobacillus plantarum.The biodiversity of isolates from Ⅲ was further investigated.At level 0.90,they were clustered into 8 genotypes.