假单胞菌L-3对氧化乐果降解条件的优化

Study on Degradating Condition of Folimat Degradation Strain Pseudomonas L-3

为确定氧化乐果降解菌假单胞菌L-3最佳的降解培养基配比及最适的环境条件,在含一定质量浓度氧化乐果的分离培养基中,对在不同碳源、氮源和磷源条件下的培养基进行降解实验,并通过正交旋转组合实验优化设计,优选出最适的碳源、氮源和磷源及其质量浓度;对菌株在不同的温度、pH值、接种体积分数、初始氧化乐果质量浓度环境条件下进行降解,通过测定降解率优化出最适的环境条件。结果表明,菌株L-3最适降解条件为:蔗糖1.7 g/dL,牛肉蛋白胨0.92 g/dL,KH2PO40.184 g/dL;最佳的降解环境条件为pH 7、30℃、接种体积分数3%、初始氧化乐果质量浓度为50 mg/L;优化后对氧化乐果的降解率高达82.59%。

The aim of this study is to determine the optimum nutrient and environmental conditions for folimat degradation by strain pseudomonas L-3,For this,the single factor experiment was applied and the optimum conditions were listed as follows： L-3 was 1.7% cane sugar,0.92% beef peptone,0.184% KH2PO4.The optimal degradation environmental condition was pH 7.0,30 ℃,3% inoculum size,50 mg/L initial folimat concentration.With the optimun conditions,the degradation rate reached at 82.59%.