高产D-乳酸生产菌株的选育

D-lactic Acid Production of High-Yielding Strains of Breeding

以凝结芽孢杆菌JD-063D为出发菌株,采用紫外线、硫酸二乙酯、钴60γ-射线辐照、微波进行复合诱变,得到正突变菌株,进行发酵检测和遗传稳定性试验,最终筛选出一株JD-76D,该菌株产酸由出发菌株的61 g/L提高到145 g/L,D-乳酸纯度由原菌株的97.5%增加到98.7%以上。

In the paper,the Bacillus coagulans JD-063 was mutagenized using UV,diethyl sulfate,cobalt 60γ-ray and microwave;Mutant strains were carried out fermentation test and genetic atability test.Finaly,we selected a strain JD-76D which had some notable features.Using the mutant strain,the amount of produced acid was increased to 145 g/L from 61 g/L using starting strain,the purity of D-lactic acid reached to 98.7% which was more than 97.5% of original strain.