bFGF对慢性酒精中毒大鼠肝组织ATP酶活力的影响

Effects of bFGF on the activities of Na^+-K^+-ATPase and Ca^(2+)-Mg^(2+)-ATPase in liver tissue of rat model of alcoholism

目的观察碱性成纤维细胞生长因子(bFGF)对慢性酒精中毒大鼠肝组织Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力的影响,探讨bFGF对慢性酒精中毒所致的肝损伤的保护作用。方法选择成年Wistar雄性大鼠,采用白酒灌胃建立慢性酒精中毒模型,慢性酒精中毒模型建立成功的大鼠随机抽签法分为酒精中毒对照组、生理盐水(NS)对照组和bFGF治疗组。另10只不灌白酒作为正常对照组。bFGF治疗组大鼠白酒灌胃的同时,1h后按12μg/kg剂量肌肉注射,共14d。各组大鼠到相对应的时间点取出肝组织制成匀浆,测定肝组织匀浆中Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力。结果与正常对照组相比,慢性酒精中毒后大鼠肝组织中Na+-K+-ATP酶活力及Ca2+-Mg2+-ATP酶活力均明显降低(P<0.05);经bFGF治疗后肝组织中Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显高于酒精中毒对照组及NS对照组(P<0.05)。结论 bFGF能提高慢性酒精中毒肝组织中Na+-K+-ATP酶活力及Ca2+-Mg2+-ATP酶活力,提示bFGF对慢性酒精中毒所致的肝损伤具有保护作用。

Objective To study the effects of basic fibroblast growth factor （bFGF） on the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in liver tissue of rat model of alcoholism.Methods The rat model of alcoholism were established by perfusing stomach with alcohol.Wistar rats were randomly divided into of model of control group,model of alcoholism group,normal saline （NS） and bFGF treatment group.The activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in liver tissue were detected.Results The activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in liver tissue were significantly decreased than those in control group （P〈0.05）.After bFGF intervention,the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in liver tissue of the bFGF group were significantly increased compared with the NS control group and model of alcoholism group respectively （P〈0.05）.Conclusion bFGF can increased the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in liver tissue in alcohol induced alcoholism model rats.