肝硬化患者血清中瘦素及可溶性瘦素受体的临床研究

Clinical study of serum leptin and Ob-R of patients with hepatocirrhosis

目的观察不同程度肝硬化患者血清中瘦素(leptin,LP)及可溶性瘦素受体(soluble leptin receptor,SLR)的水平,探讨LP及SLR在肝硬化发生发展中所起的作用及相关机制,揭示血清LP及SLR是否可作为临床评估患者肝硬化程度的非创伤性检测指标。方法清晨空腹抽血5ml,分离血清后放在-20℃冰箱中备检。用ELISA法检测血清中LP及SLR水平。结果肝硬化各级患者LP水平均较对照组明显升高,且各级间差异亦有极显著性(P<0.01),随着肝硬化严重程度进展,LP水平相对增加,而SLR较对照组明显降低,各级间差异亦有显著性(均P<0.01)。结论血清中LP及SLR测定可作为反映肝实质损伤程度的敏感指标,对肝硬化程度的判定具有重要意义。

Objective To explore the function and mechamism of leptin and soluble Ob-R in the occurrence and development of hepatocirrhosis,to evaluate seru-leptin and its receptor as one of non-invasive detection indices to assess the degree of hepatocirrhosis.Methods Phlebotomize empty 5ml in the morning,and prepared for detection in-20℃ after separating serum.Examine the level of leptin and soluble Ob-R in serum by ELISA.Results In this experiment,the leptin levels of the patients with the different class hepatocirrhosis had greater increase eased significantly than that of the controls （P〈0.01）.With the increase of the severe degree of hepatocirrhosis,the level of leptin more increased and that of soluble Ob-R more decreases significantly than those of the controls in different classes （P〈0.01）.Conclusion Serum leptin and Ob-R can be applied as the sensitive indices of injure degree of hepatocytes,especially to the assessment of the degree of hepatocirrhosis.