HGF在增生性玻璃体视网膜病变增生膜中的表达

Expression of hepatocyte growth factor in periretinal membranes of proliferative vitreoretinopathy

目的观察肝细胞生长因子(hepatocyte growth factor,HGF)在实验性增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)增生膜中的表达。方法采用玻璃体腔内注入体外培养兔视网膜色素上皮(retinal pigment epithelium,RPE)细胞法建立PVR动物模型,免疫组织化学法检测PVR增生膜中HGF的表达。结果 HGF在造模后7天已经有高表达,30天后达到高峰,60天时较30天表达略下降。结论 HGF在实验性PVR动物模型中高表达,提示HGF参与了PVR增生膜的形成。

Objective To investigate the expression of hepatocyte growth factor （HGF） in experimental animal model of periretinal membranes （PRM） of proliferative vitreoretinopathy （PVR）. Methods The retinal pigment epithelium （RPE） cells of the cultivated rabbits were separated outside their bodies,then the PVR animal model was established by injecting these RPE cells into vitreous. Using immunobistochemical technique the expression of HGF was evaluated. Results The expression of HGF in 7 days after the modeling group was higher than normal group; in 30 days it reached highest,and in 60 days it was slightly lower than than in 30 days. Conclusion Expression of HGF in periretinal membranes of experimental animal model of PVR is significantly higher than normal groups,which indicates that HGF takes part in the formation of periretinal membranes of PVR.