针刺对快速老化小鼠海马神经干细胞增殖、分化的影响

The influence of acupuncture on neural stem cell proliferation and differentiation in the hippocampus of the senescence-accelerated mouse prone-8

目的 研究针刺疗法对快速老化小鼠（SAMP8）海马神经干细胞（NSCs）增殖、分化的影响.方法 将24只SAMP8小鼠随机分为模型组与针刺组,12只抗快速老化模型小鼠（SAMR1）作为正常对照组（正常组）,针刺组选百会穴进行针刺治疗,每天治疗1次,7 d为1个疗程,共治疗3个疗程.处死前1周开始给予小鼠腹腔注射5-溴-2＇-脱氧尿苷（BrdU）,50 mg/kg体重,每日1次.动物处死后取海马组织,用免疫荧光双标方法检测NSCs的增殖、分化.结果 各组小鼠海马区都存在BrdU/nestin阳性细胞表达,与正常组比较,模型组小鼠阳性细胞数减少,差异有统计学意义（P〈0.01）;与模型组比较,针刺组小鼠阳性细胞数增多,差异有统计学意义（P〈0.05）.各组小鼠海马区都存在NSCs分化的新生神经细胞阳性表达,与模型组比较,针刺组NSCs向成熟神经元及未成熟神经元的分化不明显（P〉0.05）.针刺能使SAMP8增多的成熟星形胶质细胞表达下降,减少的未成熟星形胶质细胞表达增多,但针刺组与模型组比较,差异均无统计学意义（P〉0.05）.与正常组比较,模型组少突胶质细胞明显增多（P〈0.05）;针刺能抑制少突胶质细胞的表达,针刺组与模型组比较差异有统计学意义（P〈0.05）.结论 针刺治疗能促进SAMP8海马NSCs增殖,抑制其向少突胶质细胞分化,但对向神经元、未成熟星形胶质细胞分化的影响不明显.

Objective To investigate the influence of acupuncture therapy on neural stem ceil （NCS） prolif- eration and differentiation in the hippocampus of the senescence-accelerated prone-8 （SAP8） mouse. Methods Twenty-four SAP8 mice were randomly and equally divided into a model group and an acupuncture group. Twelve senes- cence-accelerated resistant （SAR1） mice served as the control group. Acupuncture was administered at the Baihui （DU20） point to mice in the acupuncture group once daily for 21 consecutive days. Bromodeoxyuridine （BrdU） was used to detect the proliferation and differentiation of NCSs in the hippocampus through double-labeled immunofluores- ence. Results ①n both the SAP8 group and the SAR1 group BrdU/nestin positive cells appeared. Compared to SAR1 group, the positive cells in the SAP8 group were significantly fewer. Compared to the SAP8 group, positive cells were significantly more numerous in the acupuncture group. ②In both the SAP8 group and the SAR1 group, BrdU markers of neuron or glia cell positive cells appeared. Compared to the SARI group, the expression of BrdU/GFAP in- creased in the SAP8 group, and decreased after acupuncture but not significantly. BrdU/S-100 13 cells decreased signif- icantly in the SAP8 group, and increased after acupuncture, but again not significantly. Compared to the SARI group BrdU/GalC positive cells increased significantly in the SAP8 group and decreased significantly after acupuncture. Conclusions After acupuncture treatment for 21 days, the differentiation of hippocampal NSCs into oligodendrocytes was inhibited, hat there was little effect on their differentiating into neurons and immature astrocytes.