β纤维蛋白原HaeⅢ基因多态性与2型糖尿病脑梗死的相关性研究

The Relationship between β-fibrinogen HaelII Gene Polymorphisms and Type 2 Diabetes Combined with Cerebral Infarction

目的 探讨β纤维蛋白原HaeIII基因多态性与2型糖尿病脑梗死间的关系.方法 对59名发病10d内的糖尿病合并脑梗死者（DM2CI组）、50名糖尿病患者（DM2组）和53名查体者（CON组）采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）的方法,分析受试者的基因型,用Follin酚法测定纤维蛋白原浓度.结果 各基因型频率和等位基因频率在3组之间均无显著性差异,基因型频谱P=0.713,等位基因频谱P=0.398.DM2组血浆Fg水平与DM2CI组及CON组比较,差异均有显著性（P〈0.05）.DM2CI组血浆Fg水平与CON组比较,差异无显著性（P〉0.05）.结论 未发现β纤维蛋白原HaeIII基因多态性与DM2CI 之间有显著相关性,血浆Fg水平升高可能是DM2患者脑梗死发病的危险因素之一.

Objective To investigate the relationship between β-fibrinogen HaeIII gene polymorphisms and type 2 diabetes combined with cerebral infarction. Methods One hundred and sixty-two cases were divided into diabetes mellitus combined with cerebral infarction group（DMCI group） ,diabetes mellitus group（ DM group ） and control group. The β-fibrinogen genotypes and the level of fibrinogen were detected by PCR, RFLP and Follin phenol method respectively. Results There was no significant difference among the three groups in genotype frequencies and allele frequen- cies. The fibrinogen level of DM group was higher than that of the DMCI group and control group. Conclusion The β-fibrinogen HaeIII gene polymorphisms was not found to be related with DMCI. The increase of fibrinogen level may be one of risk factors of CI for DM patients.