雷公藤内酯醇对阿尔茨海默病模型大鼠海马IL-1α表达的影响

Effect of triptolide on expression of IL-1α in the hippocampus of model rats with Alzheimer's disease

目的:探讨雷公藤内酯醇对阿尔茨海默病(AD)模型大鼠海马白细胞介素-1α(IL-1α)表达的影响。方法:30只雄性SD大鼠随机分为对照组、模型组及用药组,模型组及用药组大鼠双侧海马各一次注射Aβ1-40,对照组大鼠双侧海马注射等量生理盐水,模型制作成功后,用药组大鼠每日腹腔注射雷公藤内酯醇0.4mg/kg,共15d。采用免疫组织化学和RT-PCR方法检测IL-1α蛋白和mRNA在大鼠海马的表达。结果:免疫组织化学染色显示,模型组大鼠海马IL-1α阳性细胞数和平均光密度明显高于对照组(P<0.01),用药组大鼠海马IL-1α阳性细胞数和平均光密度较模型组明显减少(P<0.05或0.01)。RT-PCR结果显示,模型组大鼠海马IL-1αmRNA水平明显高于对照组(P<0.01),用药组大鼠海马IL-1αmRNA水平较模型组明显下降(P<0.01)。结论:雷公藤内酯醇可抑制AD模型大鼠海马IL-1α的表达。

Objective：To investigate the effect of triptolide（TP）on expression of IL-1α in the hippocampus of model rats with Alzheimer＇s disease（AD）.Methods：Thirty male SD rats were randomly divided into control group and AD model group and TP-treated group.The AD model rats were made with bilateral microinjection of aggregated beta-amyloid protein（Aβ）1-40 into hippocampus in rats and the control rats were injected with saline.The TP-treated group rats were administered TP（0.4 mg/kg·d）intraperitoneally after microinjection of Aβ1-40 into hippocampus.The expression of interleukin-1α（IL-1α）mRNA and protein of hippocampus in rats was observed with immunohistochemical staining and RT-PCR 15 days later.Results：Immunohistochemically,the cell number and average optical density of IL-1α positive staining in hippocampus of the AD model group were obviously increased as compared with the control group（P〈0.01）,the cell number and average optical density of IL-1α positive staining in the TP-treated group were obviously decreased as compared with the AD model group（P〈0.05 or 0.01）.The expression of IL-1α mRNA in hippocampus of the AD model group was obviously increased as compared with the control group（P〈0.01）.The expression of IL-1α mRNA in hippocampus of the TP-treated group was obviously decreased as compared with the AD model group（P〈0.01）.Conclusion：TP can inhibit expression of IL-1α in the hippocampus of model rats with AD.