活血祛瘀法对大鼠微动应力内固定模型早期骨痂生长的影响

Influence of Blood-activating and Stasis-dissolving Therapy on Early-stage Callus Growth in Rats with Jog Stress and Internal Fixation

目的:观察活血祛瘀法结合微动应力内固定对大鼠骨折早期骨折愈合的影响。方法:采用SD大鼠72只,造成胫骨骨折模型,随机分成治疗组,固定对照组和中药对照组,每组24只。进行术后局部大体观察及肿胀评分,同时取术后天3、5、7、10、14和21d这6个时相点,每组每时相点4只,均断颈处死后拍胫骨X线片并进行骨痂质量灰度评分。结果:在大鼠骨折术后早期3、5、7d的表现,治疗组的大体观察结果优于2个对照组。而各时相点的X线片大体观察及骨痂灰度定量分析结果显示:治疗组最早出现血肿肌化及骨痂塑形,骨痂密度高。固定组血肿肌化及骨痂出现较中药组晚,但质量高,而且良好愈合。无固定的中药组虽然骨痂出现早,量多,但是密度低、不能形成连续,骨折愈合差。结论:活血祛瘀方法和微动应力内固定对骨痂成骨有协同促进作用。

Objective：To observe the influence of blood-activating and stasis-dissolving therapy on early-stage callus growth in rats with jog stress and internal fixation.Methods：Tibia fracture models were established in 72 healthy SD rats and then randomized into treatment group,internal fixation group and Chinese medicine group,24 in each group.Observation was made to the gross appearance of wounded limb,and swelling score was graded after operation.On days 3,5,7,10,14 and 2l,4 rats were killed in each group respectively for tibia X-ray test.Results：On days 3,5,and 7,the result of the gross appearance observation of treatment group was better than that in the other two groups.Tibia X-ray photos and the callus grayscale level at each time point showed that the organization of hematoma and callus molding first appeared in treatment group,then in Chinese medicine group,and the last in internal fixation group.But quantity was better in internal fixation group,with better union.Though the organization of hematoma and callus molding of Chinese medicine group appeared earlier,they had lower density and the fracture showed poor union.Conclusion：The blood-activating and stasis-dissolving therapy may affect early-stage callus growth in rats with jog stress and internal fixation and promote the fracture healing.