电针水沟穴对脑梗死大鼠运动诱发电位的影响

Effects of electro-acupuncture at Shuigou (DU26) point on motor evoked potential in rats with cerebral infarction

目的:观察电针水沟穴对脑梗死大鼠运动诱发电位(motorevoked potential,MEP)潜伏期和波幅的影响。方法:将50只雄性健康Wistar大鼠随机分为正常组、模型组、假手术组、非穴组和水沟组,每组10只。模型组、非穴组和水沟组大鼠采用线栓法建立大脑中动脉闭塞模型,大鼠苏醒后按Zausinger六分法对其神经功能进行评分。水沟组大鼠在评分后,针刺水沟穴,并施以频率2Hz,电流1mA的连续波刺激10min,每天2次,共3d;非穴组大鼠在水沟穴左侧0.5cm针刺。72h后再次进行神经功能评分,神经电生理检测仪检测经颅电刺激MEP的潜伏期和波幅。结果:与正常组大鼠比较,模型组大鼠患侧MEP的潜伏期显著延长(P<0.05),波幅降低(P<0.01)。针刺3d后与模型组比较,水沟组大鼠患侧MEP的潜伏期明显缩短,波幅增高(P<0.05);与非穴组比较,水沟组患侧MEP的潜伏期缩短(P<0.05),波幅虽然高于非穴组,但差异无统计学意义(P>0.05)。非穴组MEP的潜伏期和波幅与模型组比较,差异无统计学意义(P>0.05)。水沟组3d后神经功能评分高于模型组和非穴组(P<0.05),模型组和非穴组神经功能评分比较,差异无统计学意义(P>0.05)。结论:电针水沟穴可以促进脑梗死大鼠MEP的恢复。

Objective：To investigate the effects of electro-acupuncture at Shuigou （DU26） on latency and amplitude of motor evoked potential （MEP） in rats after cerebral infarction.Methods：Fifty healthy male Wistar rats were randomly divided into five groups as normal group,untreated group,sham-operated group,non-acupoint group and Shuigou group,with 10 rats in each group.By using a modification of the technique of middle cerebral artery occlusion,focal cerebral ischemia injury was induced in rats except normal and sham-operated groups.Rats in the normal group received no treatment.Rats in the sham-operated group underwent identical experimental procedures as the other groups,except that the nylon suture was inserted into the internal carotid artery.After the behavioral deficit was evaluated by using Zausinger 6-point neurological function score,rats in the Shuigou group and non-acupoint group were separately acupunctured and stimulated by continuous-wave with 2 Hz frequency,1 mA intensity,for 10 minutes,twice a day for 3 days.Non-acupoint was located at the left side 0.5 cm of the Shuigou point.No intervention was given to the other groups.Zausinger 6-point neurological function and MEP were measured after 72-hour treatment.Results：The latency at the affected side in the untreated group was significantly longer than that in the normal group （P0.05）,and the amplitude at the affected side was significantly reduced （P0.01）.After 3-day electro-acupuncture,the latency at the affected side of rats in the Shuigou group was obviously shortened as compared with the untreated group （P0.05）,and the amplitude was significantly increased （P0.05）.The latency at the affected side in the Shuigou group was shorter than that in the non-acupoint group （P0.05）,and the amplitude tended to increase as compared with the non-acupoint group,but there were no significant differences （P0.05）.There were no significant differences on the latency and amplitude between the untreated group and non-acupoint group （P0.05）.The neurobehavioral score of the Shuigou group was significantly higher than those of the untreated group and non-acupoint group （P0.05）,and there were no significant differences between the untreated group and non-acupoint group （P0.05）.Conclusion：Electro-acupuncture at Shuigou can increase the latency and amplitude of MEP in rats after cerebral infarction.