HA14-1联合环磷酰胺调控肺癌小鼠Bcl-2、Bax蛋白的表达

HA14-1 Combined with Cyclophosphamide Modulates the Expression of Bcl-2 and Bax in Lung Cancer in Mice

目的:探讨HA14-1联合环磷酰胺对肺癌小鼠Bcl-2、Bax蛋白的表达。方法:建立小鼠肿瘤模型,分为生理盐水组、HA14-1组、环磷酰胺组和HA14-1+环磷酰胺组,应用Western blot和免疫组化方法检测Bcl-2、Bax的蛋白表达。结果:HA14-1组、环磷酰胺组、HA14-1+环磷酰胺组较生理盐水组Bcl-2表达减少、Bax表达增加(P<0.05),HA14-1+环磷酰胺组较单一的HA14-1组或环磷酰胺组Bcl-2表达明显减少、Bax表达明显增加(P<0.05)。结论:HA14-1可以通过抑制Bcl-2的表达,增加Bax的表达,从而促进肿瘤细胞的凋亡,同时增强环磷酰胺的抗肿瘤特性。

Objective： To explore the effect of HA14-1 combined with cyclophosphamide （CTX） on the expression of Bcl-2 and Bax proteins in lung cancer in mice. Methods： Mouse models of lung carcinoma were established and divided into 4 groups： the HA14-1 group, the CTX group, the combination HA14-1＋CTX group and the normal saline group used as the control. Western blot and immunohistochemistry were applied to detect the expression of Bcl-2 and Bax. Results： The HA14-1 group, the CTX group and the HA14-1＋CTX group had lower Bcl-2 protein expression and higher Bax expression compared with the control group （P〈0.05）. The HA14-1＋CTX group had the lowest Bcl-2 protein expression and the highest Bax protein expression （P〈0.05）. Conclusion： HA14-1 can promote tumor cell apoptosis and enhance the efficacy of CTX chemotherapy by downregulating Bcl-2 expression and upregulating Bax expression.