猪瘟病毒E_2基因疫苗表达抗原在小白鼠胫前肌的分布及消长

Distribution and Fallelevation of Hog cholera Virus E2 Gene Vaccine Expressed Antigens in the Inoculated Mouse's Anterior Tibial Muscle

用免疫组化法检测了猪瘟病毒（ＨＣＶ）Ｅ２囊膜糖蛋白基因疫苗表达的抗原在小白鼠胫前肌中的分布及消长。结果：ＨＣＶＥ２基因疫苗ｐＣＤＳＴ接种小白鼠胫前肌后，表达的Ｅ２抗原主要分布于肌纤维膜上，少量分布于肌纤维间，在细胞浆中很难检测到Ｅ２抗原；用ＨＣＶ基因疫苗免疫后１ｄ，Ｅ２抗原已有表达，１３ｄ抗原达高峰，以后逐渐减少，３０ｄ仅检测到少量抗原。

Distribution and growthdecline of Hog cholera virus (HCV) E2 envelope glucoprotein coding gene vaccine expressed antigens in the inoculated mouse's anterior tibial muscle was measured by means of immunohistochemical method. The test results revealed that after mouse's anterior tibial muscle was inoculated with HCV E2 gene vaccine pCDST the expressed E2 antigens mainly distributed in the membrances of myofibers and a few in between the myofibers, but the E2 AMTOGEMS was hard to be detected in the cytoplasm. The E2 antigens were expressed one day post inoculation of the HCV gene vaccine and reached peak value 13 days post inoculating the gene vaccine, and since then declined gradually, and a little antigen was found 30 days after the immunization.