猪圆环病毒2型的全基因克隆和序列分析

Cloning and Sequence Analysis of Complete Genome of Porcine Circovirus Type 2

参考GenBank中发表的猪圆环病毒2型（PCV-2）全基因序列,设计一对PCR引物,从病料中扩增获得4株PCV-2的全基因组序列,分别命名为SDNY-PCV-2、ShD-PCV-2、HBbd-PCV-2、GSLN-PCV-2,经测序确定长度均为1 767 bp。应用DNA Star序列分析表明,4个PCV-2分离株与国外部分分离株的核苷酸序列同源性达95.5%-99.8%,与PCV-1毒株的序列同源性为76.2%-77.6%。与国内外部分分离毒株序列进化树分析表明,4个PCV-2分离毒株处于不同分支中,存在一定差异。

According to the published genomic sequence of porcine circovirus type 2（PCV-2）,a pair of primers were designed to amplify the complete genome of PCV-2 from tissues of piglets with PMWS.Four complete genomes of PCV-2,named SDNY-PCV-2,ShD-PCV-2,HBbd-PCV-2 and GSLN-PCV-2,were amplified,respectively.The genomes of PCV-2 isolates showed that all of them were 1 767 bp in length.The four sequences were compared with other published genomes of PCVs by DNA star.The results showed that the sequences of the four isolates were closely related to those of other PCV-2 isolates in GenBank,the homologies were 95.5% to 99.8%.However,there were only 76.2% to 77.6% nucleotide the homologies with the PCV-1 isolates.The phylogenetic analysis of the domestic PCV-2 genome sequences and the foreign sequences showed that the isolated strains lied in different branches.