混合菌发酵1,3-丙二醇的初步研究

Preliminary Study on Production of 1,3-Propanediol by Mixed Bacterias

将表达酿酒酵母3-磷酸甘油脱氢酶基因(GPD1)和3-磷酸甘油酯酶基因(HOR2)的质粒PSE-gpd1-hor2转化到甘油激酶基因(glpK)和甘油脱氢酶基因(gldA)双缺失的大肠杆菌JM109C中,构建产甘油的工程菌JM109C/PSE-gpd1-hor2。接种JM109C/pSE-gpd1-hor2和Klebsiella在含1%葡萄糖的摇瓶发酵培养基中37℃发酵56h,1,3-丙二醇的最高产量为1.28g/L,葡萄糖摩尔转化率为37.5%;在30L发酵罐中发酵68h,1,3-丙二醇的最高产量为24.09g/L,葡萄糖摩尔转化率为38.0%;5g/L的乙酸、乳酸,10g/L的乙醇分别使1,3-丙二醇的产量降低了91.41%、54.68%和51.56%。

The plasmid PSE-gpd1-hor2 containing GPD1 and HOR2 from S.cerevisiae was transformed into JM109C with the deficiency of glpK and gldA.The highest yield of 1,3-propanediol in Erlenmeyer flasks from 1% glucose at 37℃ by constructed JM109C/PSE-gpd1-hor2 with Klebsiella is 1.28 g/L after 56 h and the molar conversion of glucose is 37.5%.In 30 L fermentor,the highest yield of 1,3-propanediol is 24.09 g/L and the molar conversion of glucose is 38.0% after 68 h.The by-products 5 g/L acetic acid,5 g/L lactic acid and 10 g/L ethanol can decrease the yield of 1,3-propanediol 91.41%,54.68% and 51.56%,respectively.