系统性红斑狼疮患者体内Th17、Th1细胞及其相关因子的表达及意义

Expression of Th17 and Th1 cells in peripheral blood of patients with systemic lupus erythematosus

目的 研究系统性红斑狼疮（SLE）患者血浆IL-17、干扰素（IFN）-γ水平及外周血辅助性T细胞（Th）17和Th1细胞表达比例,探讨其临床意义.方法 用酶联免疫吸附法检测SLE患者及对照组血浆中IL-17、IFN-γ浓度;运用流式细胞术检测SLE患者外周血单个核细胞（PBMCs）中Th17、Th1细胞表达比例.进一步分析Th17/Th1细胞及其相关因子与SLE临床实验室指标的相关性.结果 SLE患者PBMCs中Th17细胞比例显著高于对照组,且SLE活动期患者Th17细胞百分比高于非活动期患者（P〈0.05）,而SLE患者Th1细胞比例与对照组无差异（P〉0.05）.SLE患者血浆IL-17水平、Th17细胞数与SLE疾病活动性指数呈正相关.结论 SLE患者体内IL-17水平、Th17细胞比例显著升高,且与疾病活动性明显相关,提示IL-17/Th17细胞在SLE发病机制中可能发挥重要作用.

Objective To determine levels of plasma interleukin （IL）-17 and interferon（IFN）- γand the proportion of Th1 and Thl cells in the peripheral blood of patients with systemic lupus erythematosus（SLE）. Methods Concentrations of IL-17 and IFN-γin plasma were measured by enzyme linked immunosorbent assay. Flow cytometric assay were used to analyze the percentage of Th17 and Th1cells in SLE patients. Results The percentage of Th17 cells in patients with SLE was higher than that of normal controls and was significant increased in more active SLE patients, while there showed no statistic differences between SLE patients and normal controls for the percentage of Thl cells（ P 〉 0.05 ）. Both of plasma levels of IL-17 and the proportion of Th17 cells positively correlated with SLEDAI. Conclusion Plasma IL-17 and the percentage of Thl7 cells in SLE patients are significantly elevated and the close relationship between IL-17/Th17 cells and disease activity suggests that IL-17/Th17 may play an important role in the pathogenesis of SLE.