罗格列酮联合甘精胰岛素对骨髓基质细胞的影响

Rosiglitazone combined with insulin glargine on the impact of MSCs

目的 观察胰岛素增敏剂罗格列酮联合甘精胰岛素对骨髓基质细胞成脂分化和脂质过氧化物体（PPAR-γ）基因表达水平的影响.方法 将大鼠骨髓基质细胞分离、传代培养后分组以不同浓度药物处理,在显微镜下观察细胞形态,用油红O染色测定比色值,并用RT-PCR法检测PPAR-γ基因的表达水平.结果 用药6天,各用药组与对照组相比,均明显促进成脂分化（P〈0.05）,除甘精胰岛素10-8 mol/L组外,其他各组光密度（OD）值均明显高于成脂诱导剂组（P〈0.05）,提示高浓度甘精胰岛素和罗格列酮及其联用,均有显著的促成脂作用.各组均表达PPARγ基因,其中罗格列酮10 μmol/L组＋甘精胰岛素10-7 μmol/L组PPARγ基因表达明显增多.结论 罗格列酮与甘精胰岛素单独应用均能明显促进骨髓基质细胞的成脂分化,两者联用有协同作用.

Objective To study the effect of rosiglitazone combined with insulin glargine on the adipogenic differentiation of marrow stromal cells （MSCs） and on the expression of PPAR-γ. Methods The MSCs of rats were devided into eight groups after isolation and subculture. Then the morphology of cells was observed under microscope and the colorimetric value was determined by Oil Red O staining. The expression level of PPAR-T gene was detected by RT-PCR. Results After 6 days,compared with the control group,all the experimental groups promoted the adipogenic differentiation of bone marrow stromal cells visibly （P 〈 0.05） ,except 10-s mol/L insulin glargine group,OD values of other groups were significantly higher than the adipogenic inducer groupr（P 〈 0.05 ）, suggested that high levels of insulin glargine and rosiglitazone and combined with all had a significant role to promote fat. In addition,the expression of PPAR-γ gene was in each group and significantly increased in the 10 μmol/L rosiglitazone ＋ 10-7 mol/L insulin glarginethe group. Conclusion Rosiglitazone and insulin glargine colud evidently promote the adipogenic differentiation of MSCs when used alone. And they have a synergistic effect when used in combination.