奶牛发育各期乳腺肥大细胞的组织化学特性研究

Research on the Histochemical Properties of Mast Cells in Bovine Mammary Glands during Each Period

本研究旨在对奶牛乳腺肥大细胞的组织化学特点进行研究。对奶牛发育各期乳腺进行取样,应用改良甲苯胺蓝染色法(MTB)和阿尔新蓝—番红鉴别染色法(AB-S)进行染色,观察肥大细胞的组织化学特点和数量变化特点。同时比较了甲苯胺蓝染色时两种固定液固定对乳腺肥大细胞着染的效果。奶牛不同发育时期乳腺肥大细胞只被阿尔新蓝染色,Carnoy氏液固定效果优于中性甲醛(NBF),MTB的染色效果优于AB-S。奶牛乳腺肥大细胞的数量在泌乳各期和静止期有显著的变化。与静止期相比,泌乳期的乳腺肥大细胞数量明显减少(P<0.05),分娩后60 d奶牛乳腺肥大细胞的数量最少。奶牛乳腺只存在黏膜型肥大细胞;用Carnoy氏液固定,用MTB法染色可以很好地显示肥大细胞;泌乳期乳腺肥大细胞数量与静止期相比,明显减少。

This experiment was conducted to study the histochemical properties and change of mast cells in bovine mammary glands during each period.Specimen of mammary gland from 21 healthy dairy cow were selected and the modified toluidine blue staining（MTB） and Alcian blue-Safranin O staining（AB-S） methods were exploited to investigate the histochemical properties and change of mast cells.Meanwhile the effect of two staining methods and fixing liquid were compared.The mast cells were only stained blue by Alcian blue,the effect of Carnoy was better than that of neutral formalin（NBF）,the effect of MTB was better.Compared with non-lactating period,the number of mast cells remarkably decreased during lactating period（P0.05）.The number of mast cells was the lest in mammary gland 60 days after parturition.These results indicated that mucosal mast cells existed in mammary gland,mast cells can be shown well by fixation with Carnoy＇ liquid and staining with MTB.The number of mast cells remarkably decreased during lactating period,compared with non-lactating period.