盐酸克伦特罗胶体金快速检测试纸条的研制

Development of a Rapid Colloidal Gold Test Strip for Detecting Clenbuterol Residue

研制一种快速、简便的基于胶体金免疫层析法的试纸条,以用于对猪尿中盐酸克伦特罗的检测。以胶体金标记盐酸克伦特罗单克隆抗体竞争性膜层析技术制成了猪尿液中盐酸克伦特罗残留检测的快速检测试纸条。如待测尿样中无盐酸克伦特罗,则金标抗体能顺利地与固相载体结合,形成肉眼可见的红色带;如待测尿样中含有盐酸克伦特罗,则与金标抗体以同样的机会与固相载体结合,竞争性地占去了金标抗体与固相载体结合的机会,使金标抗体与固相载体的结合量减少,无法形成肉眼可见的红色带。经过试验制成了可检测出猪尿样中盐酸克伦特罗浓度为2 ng/mL的试纸条,可在8 min完成测试,肉眼即可判断;该试纸条灵敏度能够达到临床使用的要求,且无需任何仪器设备,具有敏感、特异、快速、简便的特点,适用于现场快速检测和筛选工作。

Developing a rapid and easy-handled test strip based on colloidal gold immunochromatographic assay,for detecting Clenbuterol residue in pig urine.The strip was successfully developed by adopting the immunochromatographic competition method.If the content of Clenbuterol residue in the sample is below a threshold quantity,most of the nanocolloidal gold-labeled anti-Clenbuterol monoclonal antibodies（Anti-CLB Mabs） bind to the solid support,forming a visible red blot.If the content of Clenbuterol residue in the pig urine is beyond the threshold quantity,Clenbuterol residue in the sample would compete with CLB-BSA for binding to the limited amount of gold-labeled Anti-CLB Mabs,they would bind to the anti-CLB Mabs in the first place,so that reducing the probability of forming Anti-CLB Mab and CLB-BSA combination,as a consequent,no visible red blot would be seen.A highly sensitive rapid colloidal gold test strip which could detect down to 2 ng/mL was successfully developed and produced;test could be done and visualized within 8 min.The sensitivity of this product has achieved the requirement for clinical use.Test could be done without any scientific instruments.The product is highly sensitive,specific,rapid,and easy-handled for rapid test,especially for on-site screening.