小鹅瘟病毒H株VP3基因序列分析

Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain

试验利用PCR技术从自行分离的GPV基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段,并与pMD18-T载体连接,转化大肠杆菌DH 5α感受态细胞,提取重组质粒经PCR和酶切鉴定后,对插入片段进行序列测定及分析。结果表明,VP3基因全长1605 bp,编码534个氨基酸;分子生物学分析表明所分离到的病毒为强毒株。

VP3 gene of goose parvovirus was amplified from the DNA by polymerase chain reaction（PCR）,the VP3 gene was cloned into pMD18-T vector.The recombinant plasmid was indentified with restriction endonuclease analysis and PCR,and then sequenced.The result of sequence analysis showed that VP3 gene had 1605 bp and included a complete open reading frame which encoding a protein of 534 amino acids,and the analysis of molecular biology showed that the virus was virulent strain.