小鼠LAK细胞对粒-单系祖细胞增殖的影响

EFFECTS OF MURINE LYMPHOKINE-ACTIVATED KILLER (LAK) CELLS ON PROLIFERATION OF MYELOID PROGENITOR CELLS (CFU-GM)

在小鼠粒单系祖细胞(CFU-GM)集落培养体系中加入LAK细胞能显著增强CFU-GM增殖,LAK∶BMC为8时,CFU-GM数比对照增加194.4%。LAK细胞条件液也有类似co-CSF的活性,单独LAK细胞条件液不能刺激CFU-GM增殖。LAK细胞和BMC共孵育4小时后再进行CFU-GM培养,低浓度LAK细胞仍能增强CFU-GM增殖,而高浓度LAK细胞则显著抑制CFU-GM增殖,LAK∶BMC为8时,CFU-GM仅为对照的27.6%。作者认为小鼠LAK细胞能通过分泌某些co-CSF增强CSF的活力,而LAK细胞对CFU-GM又有接触杀伤的活性。

Murine lymphokine-activated killer (LAK) cells were generated from spleen cells of C 57/BL 6 mice by culture of spleen cells in vitro for 72 hours in medium containing 500 units/ml recombinant human interleukin 2 (IL-2), and effects of these LAK cells on proliferation of syngenic myeloid progenitor cells (CFU-GM) were observed. After 3 days culture, LAK cells were assayed for their cytotoxicity in a 4 hours ^(51)Cr-release test. Either natural killer (NK) cell sensitive YAC-1 lymphoma cells or NK cell resistant LP-3 and WEHI-164 fibrosarcoma cells were efficiently lysed by murine LAK cells. When LAK cells were added into culture system in a final concentration of 5×10~4/ml, 2×10~5/ml, 8×10~5/ml, CFU-GM were increased by 55.2%, 165.5%, and 194.4% of control respectively. LAK-CM also showed augmentative effect on CFU-GM growth. When 10% (v/v) of LAK-CM were added into culture system, CFU-GM were increased by 51.4%0 of control, but LAK-CM alone could not stimulate CFU-GM growth. Again, effects of LAK-BMC interaction on CFU-GM formation were investigated. CFU-GM were inhibited to 27.6% of control when 1×10~5 BMC were mixed with 8×10~5 LAK cells and incubated for 4 hours prior to CFU-GM culture. These data suggest that (1) LAK cells may secrete co-CSF which showed synergistic effect with CSF on CFU-GM proliferation: (2) When LAK cells contact with BMC, they showed significant cytotoxicity to myeloid prcgenitor cells which mediated decrease of CFU-GM formation.