人和猴T淋巴细胞表面TRBC受体和E受体的比较研究

COMPARATIVE STUDY OF TRBC RECEPTOR AND E RECEPTOR ON T LYMPHOCYTES OF HUMAN AND MACAQUE

TRBC受体不同于已知的全T细胞表面分化抗原CD2,CD3/TCR复合物,CD5,CD6和CD7。CD2分子不参与TRBC玫瑰花结的形成,也不是介导E花结的唯一分子。至少有两个或两个以上蛋白质与TRBC玫瑰花结和E花结的形成有关,其中有的分子为E受体和TRBC受体所共有。因此,很可能有不同于CD2的分子参与了TRBC玫瑰花结的形成。

In 1985, rosette formation of human and macaque pan-T lymphocytes with tree shrew red blood cells (TRBC) (TRBC rosette) was first found by Ben K et al, showing different physico-chemical properties from that of rosette formation with sheep red blood cells (E-rosette). In order to approach the correlation between TRBC receptor, E receptor (CD2) and other differentiation antigens (CDs) on T lymphocytes, rosette inhibition assay and antigenic modulation or co-modulation were performed with monoclonal antibodies (McAbs) to CDs, and the distribution of TRBC receptor in other peripheral immunocytes, cell lines was also examined.TRBC rosette appeared in 88.8%of E rosette positive peripheral blood lymphocytes (E^+-PBL) and in 4.16% of E^--PBL. TRBC receptor was also found on all T cell lines tested (CEM, H 33 HJ-JA 1, Jurkat, MLA-144, Molt-3, Molt-4, Molt-4 clone 8, PEER) and some myeloid lines (U937 and HL60), but not on human granulocytes, B cell lines (Daudi, Raji and Reh) and myeloid line K 562.The modulation or co-modulation of CD 3, TCR, CDS, CD6 and CD7 with McAbs OKT3, T 108 (F 1), T 136 (F 101-15), T 149 (M-T604) and T 152 (7 G 5) did not affect TRBC rosette formation of PBL.TRBC rosette of human and rhesus monkey PBL was not inhibited by T 11. 1 McAb OKT 11 (CD 2 McAb), in contrast human and rhesus monkey E rosette formations were obviously blocked at inhibition rates of 77.9% and 49.3%, respectively. All McAbs to CD2 from the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens in Vienna 1989 were tested. T081 (X/3), T082 (CLB-T11.2/1), T083 (CLB-T11.1/1), T085 (RPA-2.10), T088 (O-275) and T 092 (M-T 201) inhibited both TRBC and E rosette formations. T 084 (F110.08), T086 (D66 clonel) (reference), T 087 (GT-2) (reference)and T 091 (AICD 2.1) only blocked E but TRBC rosette. T 090 (6F 10.3) and T198 (JOR-T2) had no effect on both rosette formations. T 084, T 086, T087, T090, T091 and OKT11 did modulate E receptor, but not TRBC receptor.These results suggest that TRBC receptor is distributed on macaque and human peripheral blood T lymphocytes and is a pan-T receptor. CD2, CD3/TCR complex, CD5, CD6 and CD7 are not involved in TRBC rosette formation. CD2 is not an unique molecule associated with E rosette formation. Two or more molecules on T cells may be involved in E or TRBC rosette formation, both share one or more molecules on T cells, and TRBC rosetting is probably associated with new molecule(s). Biochemical studies and preparation of its McAb are in progress.