接种放线菌对大豆双共生系统及其相关指标的影响

The effect of inoculation of actinomycetes on soybean dual symbiotic system and other related indicators

通过田间试验研究接种3种放线菌固体菌剂及培养基对大豆双共生系统、生物量和氮磷钾积累、土壤微生物数量、大豆产量的影响。结果表明,施用放线菌在第4片复叶展开期(V5期)与培养基处理相比对有效瘤形成、根段丛枝菌根真菌(AMF)侵染率、土壤中AMF孢子数影响不明显,结荚始期(R3期)、鼓粒盛期(R6期)放线菌处理有助于AMF孢子形成,对有效根瘤数、根段AMF侵染率的影响也受其中培养基成分的影响。放线菌处理在各时期有助于土壤细菌数量、放线菌数量的增加,各时期的放线菌数量变化趋势与土壤细菌数量的变化趋势相反,增加的土壤放线菌数量可能抑制土壤中细菌数量的增加,V5期使土壤真菌数量发生一定程度的减少,R3、R6期真菌数量迅速增加。施用放线菌在R6期对氮的积累有一定促进作用且A5菌株对植株氮积累的作用最大,只对地上部磷积累有显著促进作用,对各时期大豆生物量积累的影响较小。对大豆产量的促进作用可能是由于培养基成分引起的。

In field experiment,we studied the effects of three actinomyces and actinomycetes culture medium on soybean dual symbiotic system,content of plant nitrogen,phosphorus and potassium,soybean biomass and microbial.The results indicated that in V5 period,Inoculation of actinomycetes compared with medium,number of effective nodule,AMF infection rate and AMF spore number did not apparent increased.In R3,R6 period,actinomycetes contribute to AMF spore formation,number of effective nodule and AMF infection rate may be related to medium composition.In all periods,actinomycetes increased to bacteria number and actinomyces number in soil,but both trends was the opposite.The reason was that increaseing actinomyces number inhibited bacteria number.In V5 period,fungi number reduced,in R3,R6 period,fungi number rapidly increasing.In R6 periods,actinomyces increased accumulation of nitrogen and A5 was the best,significant increase shoots phosphorus,but in all periods,actinomyces seemed to form a little impact on soybean biomass.As the elements of culture were the reasons for increased soybean production.